private Icon ico1 = new Icon("1.ico");
private Icon ico2 = new Icon("2.ico");//透明的图标

private void button1_Click(object sender, EventArgs e)
{
timer1.Interval = 500;
timer1.Start();
}

        private void Form1_Load(object sender, EventArgs e)
{
this.notifyIcon1.Icon = ico1;
}
int i;
private void timer1_Tick(object sender, EventArgs e)
{
//如果i=0则让任务栏图标变为透明的图标并且退出
if (i < 1)
{
this.notifyIcon1.Icon = ico2;
i++;
return;
}
//如果i!=0,就让任务栏图标变为ico1,并将i置为0;
else
this.notifyIcon1.Icon = ico1;
i = 0;

        }

        private void button2_Click(object sender, EventArgs e)
{
timer1.Stop();
this.notifyIcon1.Icon = ico1;
}

        private void button3_Click(object sender, EventArgs e)
{
//if (e.CloseReason == CloseReason.UserClosing)
//{
//this.notifyIcon1.Visible = true;
//this.ShowInTaskbar = false;
//e.Cancel = true;

                //this.Hide();
notifyIcon1.Text = "系统托盘" + "\r\n";
notifyIcon1.BalloonTipTitle = "系统托盘图标闪动系统";
notifyIcon1.BalloonTipText = "O(∩_∩)O哈哈~,你好,O(∩_∩)O哈哈~,:-D";
notifyIcon1.ShowBalloonTip(1000);//消失时间
//}

        }